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SMART platform for enzyme engineering: A novel single-molecule display approach for evolution and fu...

SMART platform for enzyme engineering: A novel single-molecule display approach for evolution and fu...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_biorxiv_primary_2025_03_23_644798

SMART platform for enzyme engineering: A novel single-molecule display approach for evolution and functional studies of D-amino acid oxidase

About this item

Full title

SMART platform for enzyme engineering: A novel single-molecule display approach for evolution and functional studies of D-amino acid oxidase

Publisher

Cold Spring Harbor Laboratory

Journal title

bioRxiv, 2025-03

Language

English

Formats

Publication information

Publisher

Cold Spring Harbor Laboratory

Subjects

Subjects and topics

More information

Scope and Contents

Contents

This study introduces SMART (Single Molecule Assay on Ribonucleic Acid by Translated Product), an innovative in vitro platform integrating mRNA display, next-generation sequencing, and bioinformatics to address several key challenges in enzyme engineering. The characteristic feature of SMART is its auxiliary unit, attached to the mRNA-displayed enz...

Alternative Titles

Full title

SMART platform for enzyme engineering: A novel single-molecule display approach for evolution and functional studies of D-amino acid oxidase

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_biorxiv_primary_2025_03_23_644798

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_biorxiv_primary_2025_03_23_644798

Other Identifiers

E-ISSN

2692-8205

DOI

10.1101/2025.03.23.644798

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