低浓度亚砷酸钠下调PML蛋白表达诱导肝癌干细胞分化的分子途径
低浓度亚砷酸钠下调PML蛋白表达诱导肝癌干细胞分化的分子途径
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低浓度亚砷酸钠下调PML蛋白表达诱导肝癌干细胞分化的分子途径
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Journal title
解放军医学杂志, 2015, Vol.40 (12), p.966-971
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Chinese
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目的探讨低浓度亚砷酸钠诱导肝癌干细胞（LCSCs）分化的分子途径。方法采用免疫荧光、Western blotting和荧光定量PCR分析肝细胞癌（HCC）组织和细胞PML、Oct4和Sox2基因的表达情况,比较低浓度亚砷酸钠处理后HCC细胞PML、Oct4和Sox2基因表达及LCSCs生物功能的变化,确定低浓度亚砷酸钠对LCSCs分化的诱导作用及其分子途径。结果 0.5μg/ml亚砷酸钠可明显抑制Hu H7和原代HCC细胞形成肿瘤球,增强Hu H7细胞对吡柔吡星（THP）的敏感性（P〈0.01）,并抑制Hu H7和原代HCC细胞移植成瘤的能力（P〈0.05）。HCC组织和细胞表达PML、Oct4、Sox2m RNA和蛋白,且0.5μg/ml亚砷酸钠可使Oct4、Sox2 m RNA（P〈0....
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Full title
低浓度亚砷酸钠下调PML蛋白表达诱导肝癌干细胞分化的分子途径
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TN_cdi_chongqing_primary_667168800
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_chongqing_primary_667168800
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ISSN
0577-7402
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