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精子特异性钙离子通道(CATSPER1)蛋白在人类睾丸和射出精子中的表达及其意义

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精子特异性钙离子通道(CATSPER1)蛋白在人类睾丸和射出精子中的表达及其意义

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_chongqing_primary_689907875200603005

精子特异性钙离子通道(CATSPER1)蛋白在人类睾丸和射出精子中的表达及其意义

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Full title

精子特异性钙离子通道(CATSPER1)蛋白在人类睾丸和射出精子中的表达及其意义

Author / Creator

Hong-Gang Li Ai-Hua Liao Xiao-Fang Ding Hui Zhou Cheng-Liang Xiong Centre of Reproductive Medicine.Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030.China

Journal title

亚洲男性学杂志：英文版, 2006 (3), p.301-306

Language

English

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Contents

目的:研究精子特异性钙离子通道(CATSPER1)蛋白和 mRNA 在人类睾丸和射出精子中的表达,检测抗人 CATSPER1 抗体体外对人类精子前向运动的影响以初步探讨人 CATSPER1的功能及其作为免疫避孕靶点的可能性。方法:用逆转录聚合酶链反应(RT-PCR)和间接荧光免疫组化法分别检测液氮冻存人睾丸组织和12例正常精液标本中 CATSPER mRNA 和蛋白的表达。12例正常精液标本经 Percoll 不连续密度梯度离心法上游,上游后精子分别与终浓度为20 mg/mL、4 mg/mL、0.8 mg/mL 的抗人 CATSPER1抗体孵育,以抗体的储存液(0.01mol/L PBS,pH7.4)为对照,1 h、2 h、6 h 后用计算机辅助精液分析仪(CASA)分析前向运动精子百分率(...

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Full title

精子特异性钙离子通道(CATSPER1)蛋白在人类睾丸和射出精子中的表达及其意义

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TN_cdi_chongqing_primary_689907875200603005

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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_chongqing_primary_689907875200603005

Other Identifiers

ISSN

1008-682X

E-ISSN

1745-7262

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