A high-throughput pipeline for validation of antibodies
A high-throughput pipeline for validation of antibodies
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Publisher
New York: Nature Publishing Group US
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Language
English
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Publisher
New York: Nature Publishing Group US
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Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation...
Alternative Titles
Full title
A high-throughput pipeline for validation of antibodies
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TN_cdi_cristin_nora_10852_67592
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_cristin_nora_10852_67592
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/s41592-018-0179-8
