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An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity

An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_crossref_primary_10_7554_eLife_97543_4

An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity

About this item

Full title

An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity

Journal title

eLife, 2024-12, Vol.13

Language

English

Formats

More information

Scope and Contents

Contents

In contrast to mammalian cells, bacterial cells lack mRNA polyadenylated tails, presenting a hurdle in isolating mRNA amidst the prevalent rRNA during single-cell RNA-seq. This study introduces a novel method, ribosomal RNA-derived cDNA depletion (RiboD), seamlessly integrated into the PETRI-seq technique, yielding RiboD-PETRI. This innovative appr...

Alternative Titles

Full title

An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_crossref_primary_10_7554_eLife_97543_4

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_crossref_primary_10_7554_eLife_97543_4

Other Identifiers

ISSN

2050-084X

E-ISSN

2050-084X

DOI

10.7554/eLife.97543.4

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