Due to the high prevalence of patients attending with urinary tract infection (UTI) symptoms, the use of flow-cytometry as a rapid screening tool to avoid unnecessary cultures is becoming a widely used system in clinical practice. However, the recommended cut-points applied in flow-cytometry systems differ substantially among authors, making it difficult to obtain reliable conclusions. Here, we present FlowUTI, a shiny web-application created to establish optimal cut-off values in flow-cytometry for different UTI markers, such as bacterial or leukocyte counts, in urine from patients with UTI symptoms. This application provides a user-friendly graphical interface to perform robust statistical analysis without a specific training. Two datasets are analyzed in this manuscript: one composed of 204 urine samples from neonates and infants (≤3 months old) attended in the emergency department with suspected UTI; and the second dataset including 1174 urines samples from an elderly population attended at the primary care level. The source code is available on GitHub (
https://github.com/GuillermoMG-HUVR/Microbiology-applications/tree/FlowUTI/FlowUTI
). The web application can be executed locally from the R console. Alternatively, it can be freely accessed at
https://covidiario.shinyapps.io/flowuti/
. FlowUTI provides an easy-to-use environment for evaluating the efficiency of the urinary screening process with...

FlowUTI: An interactive web-application for optimizing the use of flow cytometry as a screening tool in urinary tract infections

10.1371/journal.pone.0277340