Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relati...
Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike‐in standard
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Publisher
England: John Wiley & Sons, Inc
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English
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Publisher
England: John Wiley & Sons, Inc
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Metabarcoding of the 16S rRNA gene is commonly used to characterize microbial communities, by estimating the relative abundance of microbes. Here, we present a method to retrieve the concentrations of the 16S rRNA gene per gram of any environmental sample using a synthetic standard in minuscule amounts (100 ppm to 1% of the 16S rRNA sequences) that...
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Full title
Absolute quantitation of microbes using 16S rRNA gene metabarcoding: A rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike‐in standard
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TN_cdi_doaj_primary_oai_doaj_org_article_0abb4dd023184476ad59e40ddc9b34e3
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_0abb4dd023184476ad59e40ddc9b34e3
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ISSN
2045-8827
E-ISSN
2045-8827
DOI
10.1002/mbo3.977
