Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutation...
Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Long-read sequencing, exemplified by PacBio, revolutionizes genomics, overcoming challenges like repetitive sequences. However, the high DNA requirement ( > 1 µg) is prohibitive for small organisms. We develop a low-input (100 ng), low-cost, and amplification-free library-generation method for PacBio sequencing (LILAP) using Tn5-based tagmentation...
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Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes
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TN_cdi_doaj_primary_oai_doaj_org_article_0b45b9eef04a4ab6983e6d97c7d3adcf
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_0b45b9eef04a4ab6983e6d97c7d3adcf
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-024-49992-6
