Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human...
Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct long-read sequencing
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Here, we develop and apply a semi-quantitative method for the high-confidence identification of pseudouridylated sites on mammalian mRNAs via direct long-read nanopore sequencing. A comparative analysis of a modification-free transcriptome reveals that the depth of coverage and specific k-mer sequences are critical parameters for accurate basecalli...
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Semi-quantitative detection of pseudouridine modifications and type I/II hypermodifications in human mRNAs using direct long-read sequencing
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TN_cdi_doaj_primary_oai_doaj_org_article_0d5179a76ab04c11a24ee5827b1cd49c
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_0d5179a76ab04c11a24ee5827b1cd49c
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-023-35858-w
