Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing...
Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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CRISPR-Cas systems inherently multiplex through CRISPR arrays—whether to defend against different invaders or mediate multi-target editing, regulation, imaging, or sensing. However, arrays remain difficult to generate due to their reoccurring repeat sequences. Here, we report a modular, one-pot scheme called CRATES to construct CRISPR arrays and ar...
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Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
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TN_cdi_doaj_primary_oai_doaj_org_article_0e7bb4e3bb974825935f7c719c17076e
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_0e7bb4e3bb974825935f7c719c17076e
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-019-10747-3
