Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins
Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins
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United States: John Wiley & Sons, Inc
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English
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United States: John Wiley & Sons, Inc
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Summary
We developed a cost sensitive isotope labelling procedure using a fed‐batch fermentation method and tested its efficiency producing the 15N‐, 13C‐ and 15N/13C‐labelled variants of an amyloidogenic miniprotein (E5: EEEAVRLYIQWLKEGGPSSGRPPPS). E5 is a surface active protein, which forms amyloids in solution. Here, we confirm, using both PM...
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Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins
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TN_cdi_doaj_primary_oai_doaj_org_article_134f0cff74d24c05a20c9df76da157bf
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_134f0cff74d24c05a20c9df76da157bf
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ISSN
1751-7915
E-ISSN
1751-7915
DOI
10.1111/1751-7915.13778