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Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins

Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_134f0cff74d24c05a20c9df76da157bf

Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins

About this item

Full title

Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins

Publisher

United States: John Wiley & Sons, Inc

Journal title

Microbial Biotechnology, 2021-05, Vol.14 (3), p.1107-1119

Language

English

Formats

Publication information

Publisher

United States: John Wiley & Sons, Inc

More information

Scope and Contents

Contents

Summary
We developed a cost sensitive isotope labelling procedure using a fed‐batch fermentation method and tested its efficiency producing the 15N‐, 13C‐ and 15N/13C‐labelled variants of an amyloidogenic miniprotein (E5: EEEAVRLYIQWLKEGGPSSGRPPPS). E5 is a surface active protein, which forms amyloids in solution. Here, we confirm, using both PM...

Alternative Titles

Full title

Bacterial fermentation and isotope labelling optimized for amyloidogenic proteins

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_134f0cff74d24c05a20c9df76da157bf

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_134f0cff74d24c05a20c9df76da157bf

Other Identifiers

ISSN

1751-7915

E-ISSN

1751-7915

DOI

10.1111/1751-7915.13778

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