Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact scr...
Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening
About this item
Full title
Author / Creator
Jin, Wei , Deng, Yexuan , La Marca, John E. , Lelliott, Emily J. , Diepstraten, Sarah T. , König, Christina , Tai, Lin , Snetkova, Valentina , Dorighi, Kristel M. , Hoberecht, Luke , Hedditch, Millicent G. , Whelan, Lauren , Healey, Geraldine , Fayle, Dan , Lau, Kieran , Potts, Margaret A. , Chen, Moore Z. , Johnston, Angus P. R. , Liao, Yang , Shi, Wei , Kueh, Andrew J. , Haley, Benjamin , Fortin, Jean-Philippe and Herold, Marco J.
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
Cas12a is a next-generation gene editing tool that enables multiplexed gene targeting. Here, we present a mouse model that constitutively expresses enhanced
Acidaminococcus sp. Cas12a
(
enAsCas12a
) linked to an mCherry fluorescent reporter. We demonstrate efficient single and multiplexed gene editing in vitro, using primary and transfo...
Alternative Titles
Full title
Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening
Authors, Artists and Contributors
Author / Creator
Deng, Yexuan
La Marca, John E.
Lelliott, Emily J.
Diepstraten, Sarah T.
König, Christina
Tai, Lin
Snetkova, Valentina
Dorighi, Kristel M.
Hoberecht, Luke
Hedditch, Millicent G.
Whelan, Lauren
Healey, Geraldine
Fayle, Dan
Lau, Kieran
Potts, Margaret A.
Chen, Moore Z.
Johnston, Angus P. R.
Liao, Yang
Shi, Wei
Kueh, Andrew J.
Haley, Benjamin
Fortin, Jean-Philippe
Herold, Marco J.
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_2b913446578449d4819602520c489f35
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_2b913446578449d4819602520c489f35
Other Identifiers
ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-025-56282-2
