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Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 cocultur...

Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 cocultur...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_2e5d8a2099744900a0518df760488daa

Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system

About this item

Full title

Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system

Publisher

Brazil: Associacao Brasileira de Divulgacao Cientifica (ABDC)

Journal title

Brazilian journal of medical and biological research, 2018-01, Vol.51 (5), p.e7183-e7183

Language

English

Formats

Publication information

Publisher

Brazil: Associacao Brasileira de Divulgacao Cientifica (ABDC)

More information

Scope and Contents

Contents

Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages of differentiation. According to the dynamic g...

Alternative Titles

Full title

Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_2e5d8a2099744900a0518df760488daa

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_2e5d8a2099744900a0518df760488daa

Other Identifiers

ISSN

0100-879X,1414-431X

E-ISSN

1414-431X,1678-4510

DOI

10.1590/1414-431x20187183

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