Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 cocultur...
Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system
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Author / Creator
Chen, Haide , Jiang, Mengmeng , Xiao, Lei and Huang, He
Publisher
Brazil: Associacao Brasileira de Divulgacao Cientifica (ABDC)
Journal title
Language
English
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Publisher
Brazil: Associacao Brasileira de Divulgacao Cientifica (ABDC)
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Scope and Contents
Contents
Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34+ cells from different stages of differentiation. According to the dynamic g...
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Full title
Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system
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Author / Creator
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Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_2e5d8a2099744900a0518df760488daa
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_2e5d8a2099744900a0518df760488daa
Other Identifiers
ISSN
0100-879X,1414-431X
E-ISSN
1414-431X,1678-4510
DOI
10.1590/1414-431x20187183