Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
About this item
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Author / Creator
Chen, Baohui , Zou, Wei , Xu, Haiyue , Liang, Ying and Huang, Bo
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
The lack of efficient tools to image non-repetitive genes in living cells has limited our ability to explore the functional impact of the spatiotemporal dynamics of such genes. Here, we addressed this issue by developing a CRISPR-Tag system using one to four highly active sgRNAs to specifically label protein-coding genes with a high signal-to-noise...
Alternative Titles
Full title
Efficient labeling and imaging of protein-coding genes in living cells using CRISPR-Tag
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Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_308d55eb091b42a9b2d29f209beb80ba
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_308d55eb091b42a9b2d29f209beb80ba
Other Identifiers
ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-018-07498-y
