Gene-targeted embryonic stem cells: real-time PCR assay for estimation of the number of neomycin sel...
Gene-targeted embryonic stem cells: real-time PCR assay for estimation of the number of neomycin selection cassettes
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England: BioMed Central Ltd
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English
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England: BioMed Central Ltd
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In the preparation of transgenic murine ES cells it is important to verify the construct has a single insertion, because an ectopic neomycin phosphortransferase positive selection cassette (
NEO
) may cause a position effect. During a recent work, where a knockin SCA28 mouse was prepared, we developed two assays based on Real-Time PCR using b...
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Full title
Gene-targeted embryonic stem cells: real-time PCR assay for estimation of the number of neomycin selection cassettes
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TN_cdi_doaj_primary_oai_doaj_org_article_32c6d9ff315c4541afeaaf36ca7ed4bc
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_32c6d9ff315c4541afeaaf36ca7ed4bc
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ISSN
1480-9222
E-ISSN
1480-9222
DOI
10.1186/1480-9222-13-10
