Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
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Publisher
London: Nature Publishing Group UK
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Language
English
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London: Nature Publishing Group UK
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Binding specificity of Cas9–guide RNA complexes to DNA is important for genome-engineering applications; however, how mismatches influence target recognition/rejection kinetics is not well understood. Here we used single-molecule FRET to probe real-time interactions between Cas9–RNA and DNA targets. The bimolecular association rate is only weakly d...
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Full title
Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
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TN_cdi_doaj_primary_oai_doaj_org_article_32ed29bb68544d38a4398de244550193
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_32ed29bb68544d38a4398de244550193
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/ncomms12778
