In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-g...
In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing
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Author / Creator
Wang, Yu , Cheng, Haijiao , Liu, Yang , Liu, Ye , Wen, Xiao , Zhang, Kun , Ni, Xiaomeng , Gao, Ning , Fan, Liwen , Zhang, Zhihui , Liu, Jiao , Chen, Jiuzhou , Wang, Lixian , Guo, Yanmei , Zheng, Ping , Wang, Meng , Sun, Jibin and Ma, Yanhe
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Reprogramming complex cellular metabolism requires simultaneous regulation of multigene expression. Ex-situ cloning-based methods are commonly used, but the target gene number and combinatorial library size are severely limited by cloning and transformation efficiencies. In-situ methods such as multiplex automated genome engineering (MAGE) depends...
Alternative Titles
Full title
In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing
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Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_33779273c5a84abd9c7115b63093df48
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_33779273c5a84abd9c7115b63093df48
Other Identifiers
ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-021-21003-y
