CRISPR technologies and the search for the PAM-free nuclease
CRISPR technologies and the search for the PAM-free nuclease
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Contents
The ever-expanding set of CRISPR technologies and their programmable RNA-guided nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with an ever-present constraint: the requirement for a protospacer adjacent motif (PAM) flanking each target. While PAMs play an essential role in self/nonself discrimination by C...
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CRISPR technologies and the search for the PAM-free nuclease
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TN_cdi_doaj_primary_oai_doaj_org_article_3b0005fc19784851be7b5e4a5d35393a
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_3b0005fc19784851be7b5e4a5d35393a
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-020-20633-y