Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
About this item
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Author / Creator
Hutchins, Elizabeth , Reiman, Rebecca , Winarta, Joseph , Beecroft, Taylor , Richholt, Ryan , De Both, Matt , Shahbander, Khalouk , Carlson, Elizabeth , Janss, Alex , Siniard, Ashley , Balak, Chris , Bruhns, Ryan , Whitsett, Timothy G. , McCoy, Roger , Anastasi, Matthew , Allen, April , Churas, Brian , Huentelman, Matthew and Van Keuren-Jensen, Kendall
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
Circular RNA (circRNA) are a recently discovered class of RNA characterized by a covalently-bonded back-splice junction. As circRNAs are inherently more stable than other RNA species, they may be detected extracellularly in peripheral biofluids and provide novel biomarkers. While circRNA have been identified previously in peripheral biofluids, there are few datasets for circRNA junctions from healthy controls. We collected 134 plasma and 114 urine samples from 54 healthy, male college athlete volunteers, and used RNASeq to determine circRNA content. The intersection of six bioinformatic tools identified 965 high-confidence, characteristic circRNA junctions in plasma and 72 in urine. Highly-expressed circRNA junctions were validated by qRT-PCR. Longitudinal samples were collected from a subset, demonstrating circRNA expression was stable over time. Lastly, the ratio of circular to linear transcripts was higher in plasma than urine. This study provides a valuable resource for characterization of circRNA in plasma and urine from healthy volunteers, one that can be developed and reassessed as researchers probe the circRNA contents of biofluids across physiological changes and disease states.
Measurement(s)
transcriptome • RNA(circular)
Technology Type(s)
RNA sequencing
Factor Type(s)
biofluid
Sample Characteristic - Organism
Homo sapiens
Machine-accessible metadata file describing the reported data:
https://doi.org/10.6084/m9.figshare.14991822...
Alternative Titles
Full title
Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
Authors, Artists and Contributors
Author / Creator
Reiman, Rebecca
Winarta, Joseph
Beecroft, Taylor
Richholt, Ryan
De Both, Matt
Shahbander, Khalouk
Carlson, Elizabeth
Janss, Alex
Siniard, Ashley
Balak, Chris
Bruhns, Ryan
Whitsett, Timothy G.
McCoy, Roger
Anastasi, Matthew
Allen, April
Churas, Brian
Huentelman, Matthew
Van Keuren-Jensen, Kendall
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_3f23b0bd131144e3863a8b03a281779c
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_3f23b0bd131144e3863a8b03a281779c
Other Identifiers
ISSN
2052-4463
E-ISSN
2052-4463
DOI
10.1038/s41597-021-01056-w