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Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes

Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_3f23b0bd131144e3863a8b03a281779c

Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes

About this item

Full title

Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes

Publisher

London: Nature Publishing Group UK

Journal title

Scientific data, 2021-10, Vol.8 (1), p.276-276, Article 276

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Circular RNA (circRNA) are a recently discovered class of RNA characterized by a covalently-bonded back-splice junction. As circRNAs are inherently more stable than other RNA species, they may be detected extracellularly in peripheral biofluids and provide novel biomarkers. While circRNA have been identified previously in peripheral biofluids, there are few datasets for circRNA junctions from healthy controls. We collected 134 plasma and 114 urine samples from 54 healthy, male college athlete volunteers, and used RNASeq to determine circRNA content. The intersection of six bioinformatic tools identified 965 high-confidence, characteristic circRNA junctions in plasma and 72 in urine. Highly-expressed circRNA junctions were validated by qRT-PCR. Longitudinal samples were collected from a subset, demonstrating circRNA expression was stable over time. Lastly, the ratio of circular to linear transcripts was higher in plasma than urine. This study provides a valuable resource for characterization of circRNA in plasma and urine from healthy volunteers, one that can be developed and reassessed as researchers probe the circRNA contents of biofluids across physiological changes and disease states.
Measurement(s)
transcriptome • RNA(circular)
Technology Type(s)
RNA sequencing
Factor Type(s)
biofluid
Sample Characteristic - Organism
Homo sapiens
Machine-accessible metadata file describing the reported data:
https://doi.org/10.6084/m9.figshare.14991822...

Alternative Titles

Full title

Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_3f23b0bd131144e3863a8b03a281779c

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_3f23b0bd131144e3863a8b03a281779c

Other Identifiers

ISSN

2052-4463

E-ISSN

2052-4463

DOI

10.1038/s41597-021-01056-w

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