DNA Extraction and Host Depletion Methods Significantly Impact and Potentially Bias Bacterial Detect...
DNA Extraction and Host Depletion Methods Significantly Impact and Potentially Bias Bacterial Detection in a Biological Fluid
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1752 N St., N.W., Washington, DC: American Society for Microbiology
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Language
English
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Publisher
1752 N St., N.W., Washington, DC: American Society for Microbiology
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Contents
Tracking the bacterial communities present in our food has the potential to inform food safety and product origin. To do so, the entire genetic material present in a sample is extracted using chemical methods or commercially available kits and sequenced using next-generation platforms to provide a snapshot of the microbial composition.
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DNA Extraction and Host Depletion Methods Significantly Impact and Potentially Bias Bacterial Detection in a Biological Fluid
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TN_cdi_doaj_primary_oai_doaj_org_article_40a0a49ba60f47f68a9cb5dc661ed0d7
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_40a0a49ba60f47f68a9cb5dc661ed0d7
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ISSN
2379-5077
E-ISSN
2379-5077
DOI
10.1128/mSystems.00619-21