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Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choic...

Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choic...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_42b3edb4b2d44bb1aadf58f87712c93c

Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

About this item

Full title

Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

Publisher

England: BioMed Central Ltd

Journal title

BMC immunology, 2010-05, Vol.11 (1), p.21-21

Language

English

Formats

Publication information

Publisher

England: BioMed Central Ltd

More information

Scope and Contents

Contents

Gene expression in lipopolysaccharide (LPS)-stimulated monocytes is mainly studied by quantitative real-time reverse transcription PCR (RT-qPCR) using GAPDH (glyceraldehyde 3-phosphate dehydrogenase) or ACTB (beta-actin) as reference gene for normalization. Expression of traditional reference genes has been shown to vary substantially under certain...

Alternative Titles

Full title

Gene expression results in lipopolysaccharide-stimulated monocytes depend significantly on the choice of reference genes

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_42b3edb4b2d44bb1aadf58f87712c93c

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_42b3edb4b2d44bb1aadf58f87712c93c

Other Identifiers

ISSN

1471-2172

E-ISSN

1471-2172

DOI

10.1186/1471-2172-11-21

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