Absolute protein quantification using fluorescence measurements with FPCountR
Absolute protein quantification using fluorescence measurements with FPCountR
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
This paper presents a generalisable method for the calibration of fluorescence readings on microplate readers, in order to convert arbitrary fluorescence units into absolute units. FPCountR relies on the generation of bespoke fluorescent protein (FP) calibrants, assays to determine protein concentration and activity, and a corresponding analytical...
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Full title
Absolute protein quantification using fluorescence measurements with FPCountR
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TN_cdi_doaj_primary_oai_doaj_org_article_4edadef23ba44761a2cb505d298ed621
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_4edadef23ba44761a2cb505d298ed621
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-022-34232-6
