Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase inte...
Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates
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Publisher
London: Nature Publishing Group UK
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Language
English
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London: Nature Publishing Group UK
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The combination of ultrahigh-throughput screening and sequencing informs on function and intragenic epistasis within combinatorial protein mutant libraries. Establishing a droplet-based, in vitro compartmentalised approach for robust expression and screening of protein kinase cascades (>10
7
variants/day) allowed us to dissect the intrinsic m...
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Full title
Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates
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TN_cdi_doaj_primary_oai_doaj_org_article_5d041683bf7f4218bf7e710dc897fb55
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_5d041683bf7f4218bf7e710dc897fb55
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-022-28396-4
