Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity
Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity
About this item
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Author / Creator
Fu, Rongjie , He, Wei , Dou, Jinzhuang , Villarreal, Oscar D. , Bedford, Ella , Wang, Helen , Hou, Connie , Zhang, Liang , Wang, Yalong , Ma, Dacheng , Chen, Yiwen , Gao, Xue , Depken, Martin and Xu, Han
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
The specificity of CRISPR/Cas9 genome editing is largely determined by the sequences of guide RNA (gRNA) and the targeted DNA, yet the sequence-dependent rules underlying off-target effects are not fully understood. To systematically explore the sequence determinants governing CRISPR/Cas9 specificity, here we describe a dual-target system to measur...
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Full title
Systematic decomposition of sequence determinants governing CRISPR/Cas9 specificity
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TN_cdi_doaj_primary_oai_doaj_org_article_5fc93d0311ad442eb76be261e9de5028
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_5fc93d0311ad442eb76be261e9de5028
Other Identifiers
ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-022-28028-x
