Maximizing Cumulative Trypsin Activity with Calcium at Elevated Temperature for Enhanced Bottom-Up P...
Maximizing Cumulative Trypsin Activity with Calcium at Elevated Temperature for Enhanced Bottom-Up Proteome Analysis
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Basel: MDPI AG
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Language
English
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Basel: MDPI AG
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Contents
Bottom-up proteomics relies on efficient trypsin digestion ahead of MS analysis. Prior studies have suggested digestion at elevated temperature to accelerate proteolysis, showing an increase in the number of MS-identified peptides. However, improved sequence coverage may be a consequence of partial digestion, as higher temperatures destabilize and...
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Full title
Maximizing Cumulative Trypsin Activity with Calcium at Elevated Temperature for Enhanced Bottom-Up Proteome Analysis
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TN_cdi_doaj_primary_oai_doaj_org_article_638cec0e99b24424b35d5f4380fb657f
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_638cec0e99b24424b35d5f4380fb657f
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ISSN
2079-7737
E-ISSN
2079-7737
DOI
10.3390/biology11101444
