CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption...
CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
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Author / Creator
Kluesner, Mitchell G. , Lahr, Walker S. , Lonetree, Cara-lin , Smeester, Branden A. , Qiu, Xiaohong , Slipek, Nicholas J. , Claudio Vázquez, Patricia N. , Pitzen, Samuel P. , Pomeroy, Emily J. , Vignes, Madison J. , Lee, Samantha C. , Bingea, Samuel P. , Andrew, Aneesha A. , Webber, Beau R. and Moriarity, Branden S.
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London: Nature Publishing Group UK
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Language
English
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London: Nature Publishing Group UK
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Contents
CRISPR-Cas9 cytidine and adenosine base editors (CBEs and ABEs) can disrupt genes without introducing double-stranded breaks by inactivating splice sites (BE-splice) or by introducing premature stop (pmSTOP) codons. However, no in-depth comparison of these methods or a modular tool for designing BE-splice sgRNAs exists. To address these needs, we develop SpliceR (
http://z.umn.edu/spliceR
) to design and rank BE-splice sgRNAs for any Ensembl annotated genome, and compared disruption approaches in T cells using a screen against the TCR-CD3 MHC Class I immune synapse. Among the targeted genes, we find that targeting splice-donors is the most reliable disruption method, followed by targeting splice-acceptors, and introducing pm...
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Full title
CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
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TN_cdi_doaj_primary_oai_doaj_org_article_66661828f7814bdf8c8c049d29a4b13b
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_66661828f7814bdf8c8c049d29a4b13b
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ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-021-22009-2
