Next generation genetically encoded fluorescent sensors for serotonin
Next generation genetically encoded fluorescent sensors for serotonin
About this item
Full title
Author / Creator
Kubitschke, Martin , Müller, Monika , Wallhorn, Lutz , Pulin, Mauro , Mittag, Manuel , Pollok, Stefan , Ziebarth, Tim , Bremshey, Svenja , Gerdey, Jill , Claussen, Kristin Carolin , Renken, Kim , Groß, Juliana , Gneiße, Pascal , Meyer, Niklas , Wiegert, J. Simon , Reiner, Andreas , Fuhrmann, Martin and Masseck, Olivia Andrea
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
We developed a family of genetically encoded serotonin (5-HT) sensors (sDarken) on the basis of the native 5-HT1A receptor and circularly permuted GFP. sDarken 5-HT sensors are bright in the unbound state and diminish their fluorescence upon binding of 5-HT. Sensor variants with different affinities for serotonin were engineered to increase the ver...
Alternative Titles
Full title
Next generation genetically encoded fluorescent sensors for serotonin
Authors, Artists and Contributors
Author / Creator
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_67b7ba204f424e40b9234b7c93a4305b
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_67b7ba204f424e40b9234b7c93a4305b
Other Identifiers
ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-022-35200-w
