Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow...
Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow cytometry
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Publisher
United States: Public Library of Science
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Language
English
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United States: Public Library of Science
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Contents
A simple method for extraction of high quality RNA from cells that have been fixed, stained and sorted by flow cytometry would allow routine transcriptome analysis of highly purified cell populations and single cells. However, formaldehyde fixation impairs RNA extraction and inhibits RNA amplification. Here we show that good quality RNA can be read...
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Full title
Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow cytometry
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TN_cdi_doaj_primary_oai_doaj_org_article_6c5ebb68944d4aba816ffb49d6527baa
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_6c5ebb68944d4aba816ffb49d6527baa
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ISSN
1932-6203
E-ISSN
1932-6203
DOI
10.1371/journal.pone.0240769
