Using the Volta phase plate with defocus for cryo-EM single particle analysis
Using the Volta phase plate with defocus for cryo-EM single particle analysis
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Publisher
England: eLife Science Publications, Ltd
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English
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England: eLife Science Publications, Ltd
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Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to include a small amount of defocus which enables contrast transfer function measurement and correction. This hybrid approach simplifies the experiment and increases...
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Full title
Using the Volta phase plate with defocus for cryo-EM single particle analysis
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TN_cdi_doaj_primary_oai_doaj_org_article_6d5fa16d07af489e9e88ae9e90d43728
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_6d5fa16d07af489e9e88ae9e90d43728
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ISSN
2050-084X
E-ISSN
2050-084X
DOI
10.7554/eLife.23006
