Improved library preparation protocols for amplicon sequencing-based noninvasive fetal genotyping fo...
Improved library preparation protocols for amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen-negative alleles
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Publisher
London: Springer Science and Business Media LLC
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Language
English
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Publisher
London: Springer Science and Business Media LLC
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Contents
We aimed to simplify our fetal RHD genotyping protocol by changing the method to attach Illumina's sequencing adaptors to PCR products from the ligation-based method to a PCR-based method, and to improve its reliability and robustness by introducing unique molecular indexes, which allow us to count the numbers of DNA fragments used as PCR templates...
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Full title
Improved library preparation protocols for amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen-negative alleles
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TN_cdi_doaj_primary_oai_doaj_org_article_6ef6fc351f874c568de86fe1c3e83f01
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_6ef6fc351f874c568de86fe1c3e83f01
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ISSN
1756-0500
E-ISSN
1756-0500
DOI
10.1186/s13104-021-05793-4
