Efficient targeted integration directed by short homology in zebrafish and mammalian cells
Efficient targeted integration directed by short homology in zebrafish and mammalian cells
About this item
Full title
Author / Creator
Wierson, Wesley A , Welker, Jordan M , Almeida, Maira P , Mann, Carla M , Webster, Dennis A , Torrie, Melanie E , Weiss, Trevor J , Kambakam, Sekhar , Vollbrecht, Macy K , Lan, Merrina , McKeighan, Kenna C , Levey, Jacklyn , Ming, Zhitao , Wehmeier, Alec , Mikelson, Christopher S , Haltom, Jeffrey A , Kwan, Kristen M , Chien, Chi-Bin , Balciunas, Darius , Ekker, Stephen C , Clark, Karl J , Webber, Beau R , Moriarity, Branden S , Solin, Stacy L , Carlson, Daniel F , Dobbs, Drena L , McGrail, Maura and Essner, Jeffrey
Publisher
England: eLife Science Publications, Ltd
Journal title
Language
English
Formats
Publication information
Publisher
England: eLife Science Publications, Ltd
Subjects
More information
Scope and Contents
Contents
Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses short homology of 24-48 bp to drive targeted integra...
Alternative Titles
Full title
Efficient targeted integration directed by short homology in zebrafish and mammalian cells
Authors, Artists and Contributors
Author / Creator
Welker, Jordan M
Almeida, Maira P
Mann, Carla M
Webster, Dennis A
Torrie, Melanie E
Weiss, Trevor J
Kambakam, Sekhar
Vollbrecht, Macy K
Lan, Merrina
McKeighan, Kenna C
Levey, Jacklyn
Ming, Zhitao
Wehmeier, Alec
Mikelson, Christopher S
Haltom, Jeffrey A
Kwan, Kristen M
Chien, Chi-Bin
Balciunas, Darius
Ekker, Stephen C
Clark, Karl J
Webber, Beau R
Moriarity, Branden S
Solin, Stacy L
Carlson, Daniel F
Dobbs, Drena L
McGrail, Maura
Essner, Jeffrey
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_753d782fc25e478bab0a05b01b9d9f77
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_753d782fc25e478bab0a05b01b9d9f77
Other Identifiers
ISSN
2050-084X
E-ISSN
2050-084X
DOI
10.7554/eLife.53968
