Selection of chromosomal DNA libraries using a multiplex CRISPR system
Selection of chromosomal DNA libraries using a multiplex CRISPR system
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Author / Creator
Ryan, Owen W , Skerker, Jeffrey M , Maurer, Matthew J , Li, Xin , Tsai, Jordan C , Poddar, Snigdha , Lee, Michael E , DeLoache, Will , Dueber, John E , Arkin, Adam P , Cate, Jamie HD and Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
Publisher
England: eLife Sciences Publications Ltd
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Language
English
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Publisher
England: eLife Sciences Publications Ltd
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Contents
The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number effects, or the use of complex marker-dependent chromosomal integr...
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Full title
Selection of chromosomal DNA libraries using a multiplex CRISPR system
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TN_cdi_doaj_primary_oai_doaj_org_article_8514d54beee74222afc3449982485145
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_8514d54beee74222afc3449982485145
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ISSN
2050-084X
E-ISSN
2050-084X
DOI
10.7554/eLife.03703
