One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes
One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes
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Publisher
England: BioMed Central Ltd
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Language
English
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Publisher
England: BioMed Central Ltd
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The homologous recombination (HR) pathway is largely inactive in early embryos prior to the first cell division, making it difficult to achieve targeted gene knock-ins. The homology-mediated end joining (HMEJ)-based strategy has been shown to increase knock-in efficiency relative to HR, non-homologous end joining (NHEJ), and microhomology-mediated...
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Full title
One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes
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TN_cdi_doaj_primary_oai_doaj_org_article_8631b0fe8f854c6b809a56145bbc2a7d
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_8631b0fe8f854c6b809a56145bbc2a7d
Other Identifiers
ISSN
1471-2164
E-ISSN
1471-2164
DOI
10.1186/s12864-021-07418-3
