A multiparametric fluorescence assay for screening aptamer–protein interactions based on microbeads
A multiparametric fluorescence assay for screening aptamer–protein interactions based on microbeads
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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For improving aptamer-ligand binding we have developed a screening system that defines optimal binding buffer composition. Using multiplex assays, one buffer system is needed which guarantees the specific binding of all aptamers. We investigated nine peer-reviewed DNA aptamers. Non-specific binding of aptamers is an obstacle. To address this, we in...
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A multiparametric fluorescence assay for screening aptamer–protein interactions based on microbeads
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TN_cdi_doaj_primary_oai_doaj_org_article_88e2442d75234fd1b5a56c6b7976df8d
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_88e2442d75234fd1b5a56c6b7976df8d
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-022-06817-0
