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Improved freshwater macroinvertebrate detection from environmental DNA through minimized nontarget a...

Improved freshwater macroinvertebrate detection from environmental DNA through minimized nontarget a...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_8c86039676b44201bbfd91e5e5f13f52

Improved freshwater macroinvertebrate detection from environmental DNA through minimized nontarget amplification

About this item

Full title

Improved freshwater macroinvertebrate detection from environmental DNA through minimized nontarget amplification

Publisher

Hoboken: John Wiley & Sons, Inc

Journal title

Environmental DNA (Hoboken, N.J.), 2021-01, Vol.3 (1), p.261-276

Language

English

Formats

Publication information

Publisher

Hoboken: John Wiley & Sons, Inc

More information

Scope and Contents

Contents

DNA metabarcoding of freshwater communities typically relies on PCR amplification of a fragment of the mitochondrial cytochrome c oxidase I (COI) gene with degenerate primers. The advantage of COI is its taxonomic resolution and the availability of an extensive reference database. However, when universal primers are used on environmental DNA (eDNA)...

Alternative Titles

Full title

Improved freshwater macroinvertebrate detection from environmental DNA through minimized nontarget amplification

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_8c86039676b44201bbfd91e5e5f13f52

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_8c86039676b44201bbfd91e5e5f13f52

Other Identifiers

ISSN

2637-4943

E-ISSN

2637-4943

DOI

10.1002/edn3.177

How to access this item