Host DNA depletion on frozen human respiratory samples enables successful metagenomic sequencing for...
Host DNA depletion on frozen human respiratory samples enables successful metagenomic sequencing for microbiome studies
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Most respiratory microbiome studies use amplicon sequencing due to high host DNA. Metagenomics sequencing offers finer taxonomic resolution, phage assessment, and functional characterization. We evaluated five host DNA depletion methods on frozen nasal swabs from healthy adults, sputum from people with cystic fibrosis (pwCF), and bronchoalveolar la...
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Host DNA depletion on frozen human respiratory samples enables successful metagenomic sequencing for microbiome studies
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TN_cdi_doaj_primary_oai_doaj_org_article_904329c9b9e5410ca04d6843f79769a1
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_904329c9b9e5410ca04d6843f79769a1
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ISSN
2399-3642
E-ISSN
2399-3642
DOI
10.1038/s42003-024-07290-3
