Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characteri...
Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunity
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Author / Creator
Hoving, Dennis , Marques, Alexandre H. C. , Huisman, Wesley , Nosoh, Beckley A. , de Kroon, Alicia C. , van Hengel, Oscar R. J. , Wu, Bing-Ru , Steenbergen, Rosanne A. M. , van Helden, Pauline M. , Urban, Britta C. , Dhar, Nisha , Ferreira, Daniela M. , Kwatra, Gaurav , Hokke, Cornelis H. and Jochems, Simon P.
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publication information
Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
Bacterial capsular polysaccharides are important vaccine immunogens. However, the study of polysaccharide-specific immune responses has been hindered by technical restrictions. Here, we developed and validated a high-throughput method to analyse antigen-specific B cells using combinatorial staining with fluorescently-labelled capsular polysaccharid...
Alternative Titles
Full title
Combinatorial multimer staining and spectral flow cytometry facilitate quantification and characterization of polysaccharide-specific B cell immunity
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Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_9cc08f4b95f4401d8d74a185a887fc67
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_9cc08f4b95f4401d8d74a185a887fc67
Other Identifiers
ISSN
2399-3642
E-ISSN
2399-3642
DOI
10.1038/s42003-023-05444-3
