An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity
An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity
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England: eLife Sciences Publications, Ltd
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English
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England: eLife Sciences Publications, Ltd
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In contrast to mammalian cells, bacterial cells lack mRNA polyadenylated tails, presenting a hurdle in isolating mRNA amidst the prevalent rRNA during single-cell RNA-seq. This study introduces a novel method, ribosomal RNA-derived cDNA depletion (RiboD), seamlessly integrated into the PETRI-seq technique, yielding RiboD-PETRI. This innovative appr...
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An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity
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TN_cdi_doaj_primary_oai_doaj_org_article_9e53c0c901c340ecb59a719561f6919c
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_9e53c0c901c340ecb59a719561f6919c
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ISSN
2050-084X
E-ISSN
2050-084X
DOI
10.7554/eLife.97543