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Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on...

Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_9f5e4001803e45c99f3b5822fd1097f3

Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on GFP chromophore

About this item

Full title

Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on GFP chromophore

Publisher

London: Nature Publishing Group UK

Journal title

Communications biology, 2022-07, Vol.5 (1), p.706-10, Article 706

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

“Fluorescence-Activating and absorption-Shifting Tag” (FAST) is a well-studied fluorogen-activating protein with high brightness and low size, able to activate a wide range of fluorogens. This makes FAST a promising target for both protein and fluorogen optimization. Here, we describe the structure-based rational design of the enhanced FAST mutants...

Alternative Titles

Full title

Structure-based rational design of an enhanced fluorogen-activating protein for fluorogens based on GFP chromophore

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_9f5e4001803e45c99f3b5822fd1097f3

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_9f5e4001803e45c99f3b5822fd1097f3

Other Identifiers

ISSN

2399-3642

E-ISSN

2399-3642

DOI

10.1038/s42003-022-03662-9

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