Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
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Publisher
England: BioMed Central Ltd
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Language
English
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Publisher
England: BioMed Central Ltd
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Contents
We report that engineered Cas9 variants with improved specificity-eCas9-1.1 and Cas9-HF1-are often poorly active in human cells, when complexed with single guide RNAs (sgRNAs) with a mismatch at the 5' terminus, relative to target DNA sequences. Because the nucleotide at the 5' end of sgRNAs, expressed under the control of the commonly-used U6 prom...
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Full title
Rescue of high-specificity Cas9 variants using sgRNAs with matched 5’ nucleotides
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TN_cdi_doaj_primary_oai_doaj_org_article_a538a35628144034aace727b8f5df66c
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_a538a35628144034aace727b8f5df66c
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ISSN
1474-760X,1474-7596
E-ISSN
1474-760X
DOI
10.1186/s13059-017-1355-3
