Improved selection of zebrafish CRISPR editing by early next-generation sequencing based genotyping
Improved selection of zebrafish CRISPR editing by early next-generation sequencing based genotyping
About this item
Full title
Author / Creator
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
Despite numerous prior attempts to improve knock-in (KI) efficiency, the introduction of precise base pair substitutions by the CRISPR-Cas9 technique in zebrafish remains challenging. In our efforts to generate KI zebrafish models of human
CACNA1C
mutations, we have tested the effect of several CRISPR determinants on KI efficiency across two...
Alternative Titles
Full title
Improved selection of zebrafish CRISPR editing by early next-generation sequencing based genotyping
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_a80db3a6daa9481ea82d1423965402c5
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_a80db3a6daa9481ea82d1423965402c5
Other Identifiers
ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-023-27503-9
