Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in me...
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Exogenous pathway optimization and chassis engineering are two crucial methods for heterologous pathway expression. The two methods are normally carried out step-wise and in a trial-and-error manner. Here we report a recombinase-based combinatorial method (termed “SCRaMbLE-in”) to tackle both challenges simultaneously. SCRaMbLE-in includes an in vi...
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Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods
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TN_cdi_doaj_primary_oai_doaj_org_article_ad81529cd72f4cf5b421bbb08fbc1ca6
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_ad81529cd72f4cf5b421bbb08fbc1ca6
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2041-1723
E-ISSN
2041-1723
DOI
10.1038/s41467-018-04254-0
