Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni
Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni
About this item
Full title
Author / Creator
Ittiprasert, Wannaporn , Mann, Victoria H , Karinshak, Shannon E , Coghlan, Avril , Rinaldi, Gabriel , Sankaranarayanan, Geetha , Chaidee, Apisit , Tanno, Toshihiko , Kumkhaek, Chutima , Prangtaworn, Pannathee , Mentink-Kane, Margaret M , Cochran, Christina J , Driguez, Patrick , Holroyd, Nancy , Tracey, Alan , Rodpai, Rutchanee , Everts, Bart , Hokke, Cornelis H , Hoffmann, Karl F , Berriman, Matthew and Brindley, Paul J
Publisher
England: eLife Sciences Publications Ltd
Journal title
Language
English
Formats
Publication information
Publisher
England: eLife Sciences Publications Ltd
Subjects
More information
Scope and Contents
Contents
CRISPR/Cas9-based genome editing has yet to be reported in species of the Platyhelminthes. We tested this approach by targeting omega-1 (ω1) of Schistosoma mansoni as proof of principle. This secreted ribonuclease is crucial for Th2 polarization and granuloma formation. Schistosome eggs were exposed to Cas9 complexed with guide RNA complementary to...
Alternative Titles
Full title
Programmed genome editing of the omega-1 ribonuclease of the blood fluke, Schistosoma mansoni
Authors, Artists and Contributors
Author / Creator
Mann, Victoria H
Karinshak, Shannon E
Coghlan, Avril
Rinaldi, Gabriel
Sankaranarayanan, Geetha
Chaidee, Apisit
Tanno, Toshihiko
Kumkhaek, Chutima
Prangtaworn, Pannathee
Mentink-Kane, Margaret M
Cochran, Christina J
Driguez, Patrick
Holroyd, Nancy
Tracey, Alan
Rodpai, Rutchanee
Everts, Bart
Hokke, Cornelis H
Hoffmann, Karl F
Berriman, Matthew
Brindley, Paul J
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_b44d0fcb161541f696572c51b67e0c12
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_b44d0fcb161541f696572c51b67e0c12
Other Identifiers
ISSN
2050-084X
E-ISSN
2050-084X
DOI
10.7554/eLife.41337
