Optimizing Systems for Cas9 Expression in <named-content content-type="genus-species">Toxoplasma gon...
Optimizing Systems for Cas9 Expression in <named-content content-type="genus-species">Toxoplasma gondii</named-content>
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American Society for Microbiology
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English
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American Society for Microbiology
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ABSTRACT CRISPR-Cas9 technologies have enabled genome engineering in an unprecedented array of species, accelerating biological studies in both model and nonmodel systems. However, Cas9 can be inherently toxic, which has limited its use in some organisms. We previously described the serendipitous discovery of a single guide RNA (sgRNA) that helped...
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Optimizing Systems for Cas9 Expression in
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TN_cdi_doaj_primary_oai_doaj_org_article_b50bb84dea504b1da97dce9b939b4eab
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_b50bb84dea504b1da97dce9b939b4eab
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2379-5042
DOI
10.1128/mSphere.00386-19