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Optimizing Systems for Cas9 Expression in <named-content content-type="genus-species">Toxoplasma gon...

Optimizing Systems for Cas9 Expression in <named-content content-type="genus-species">Toxoplasma gon...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_b50bb84dea504b1da97dce9b939b4eab

Optimizing Systems for Cas9 Expression in <named-content content-type="genus-species">Toxoplasma gondii</named-content>

About this item

Full title

Optimizing Systems for Cas9 Expression in <named-content content-type="genus-species">Toxoplasma gondii</named-content>

Publisher

American Society for Microbiology

Journal title

mSphere, 2019-06, Vol.4 (3)

Language

English

Formats

Publication information

Publisher

American Society for Microbiology

More information

Scope and Contents

Contents

ABSTRACT CRISPR-Cas9 technologies have enabled genome engineering in an unprecedented array of species, accelerating biological studies in both model and nonmodel systems. However, Cas9 can be inherently toxic, which has limited its use in some organisms. We previously described the serendipitous discovery of a single guide RNA (sgRNA) that helped...

Alternative Titles

Full title

Optimizing Systems for Cas9 Expression in Toxoplasma gondii

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_doaj_primary_oai_doaj_org_article_b50bb84dea504b1da97dce9b939b4eab

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_b50bb84dea504b1da97dce9b939b4eab

Other Identifiers

E-ISSN

2379-5042

DOI

10.1128/mSphere.00386-19

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