An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and...
An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
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Publisher
London: Nature Publishing Group UK
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Language
English
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London: Nature Publishing Group UK
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Green fluorescent proteins (GFPs) are widely used to monitor membrane protein expression, purification, and stability. An ideal reporter should be stable itself and provide high sensitivity and yield. Here, we demonstrate that a coral (
Galaxea fascicularis
) thermostable GFP (TGP) is by such reasons an improved tag compared to the convention...
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Full title
An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification
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TN_cdi_doaj_primary_oai_doaj_org_article_baab4a34e2bb410bbf23d2caba607e37
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_baab4a34e2bb410bbf23d2caba607e37
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ISSN
2399-3642
E-ISSN
2399-3642
DOI
10.1038/s42003-020-01478-z
