Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species...
Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome
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United States: American Society for Microbiology
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Language
English
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Publisher
United States: American Society for Microbiology
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Contents
Microbiome studies primarily use 16S rRNA gene amplicon sequencing to assess the relative abundance of bacterial taxa in a community. However, these measurements do not accurately reflect absolute taxon concentrations. We sought to determine whether the product of species’ relative abundance and total bacterial load measured by broad-range qPCR is...
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Full title
Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome
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TN_cdi_doaj_primary_oai_doaj_org_article_bdad9b0c0bff482d853a48dc2e675993
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_bdad9b0c0bff482d853a48dc2e675993
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ISSN
2379-5077
E-ISSN
2379-5077
DOI
10.1128/mSystems.00777-19
