Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce...
Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms
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United States: American Society for Microbiology
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Language
English
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Publisher
United States: American Society for Microbiology
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Contents
Leishmania
parasites cause human leishmaniasis. To accelerate characterization of
Leishmania
genes for new drug and vaccine development, we optimized and simplified the CRISPR-Cas9 genome-editing tool for
Leishmania
. We show that co-CRISPR targeting of the miltefosine transporter gene and serial transfections of an oligonucleotide d...
Alternative Titles
Full title
Optimized CRISPR-Cas9 Genome Editing for Leishmania and Its Use To Target a Multigene Family, Induce Chromosomal Translocation, and Study DNA Break Repair Mechanisms
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TN_cdi_doaj_primary_oai_doaj_org_article_c2a6be188d9c4d9ca3e338b03e97bfb0
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_c2a6be188d9c4d9ca3e338b03e97bfb0
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ISSN
2379-5042
E-ISSN
2379-5042
DOI
10.1128/mSphere.00340-16
