Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherich...
Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli
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London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Bovine enterokinase light chain (EK
L
) is an industrially useful protease for accurate removal of affinity-purification tags from high-value biopharmaceuticals. However, recombinant expression in
Escherichia coli
produces insoluble inclusion bodies, requiring solubilisation, refolding, and autocatalytic activation to recover functional...
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Full title
Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli
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TN_cdi_doaj_primary_oai_doaj_org_article_c73df12f10b54e7cba3c138d6b532ca1
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_c73df12f10b54e7cba3c138d6b532ca1
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-022-22574-6
