A versatile and efficient method to isolate nuclei from low-input cryopreserved tissues for single-n...
A versatile and efficient method to isolate nuclei from low-input cryopreserved tissues for single-nuclei transcriptomics
About this item
Full title
Author / Creator
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
Clinical samples are vital for understanding diseases, but their scarcity requires refined research methods. Emerging single-cell technologies offer detailed views of tissue heterogeneity but need sufficient fully characterized tissues. We developed an optimized single-nuclei RNA sequencing (snRNA-seq) protocol to extract nuclei from just 15 mg of...
Alternative Titles
Full title
A versatile and efficient method to isolate nuclei from low-input cryopreserved tissues for single-nuclei transcriptomics
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_doaj_primary_oai_doaj_org_article_c85ca1e2269a470882751f4dbfcf98dd
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_c85ca1e2269a470882751f4dbfcf98dd
Other Identifiers
ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-025-90070-8
