AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design
AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design
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England: BioMed Central Ltd
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English
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England: BioMed Central Ltd
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The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distinguishable by sgRNA designing tools based on one reference genome. AlleleAnalyzer is an open-source software that incorporates single-nucleotide variants and short insertions and deletions to design sgRNAs for precisely editing 1 or multiple haplotypes of a sequenced genome, currently supporting 11 Cas proteins. It also leverages patterns of shared genetic variation to optimize sgRNA design for different human populations. AlleleAnalyzer is available at https://github.com/keoughkath/AlleleAnalyzer ....
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AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design
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TN_cdi_doaj_primary_oai_doaj_org_article_caf4e3785dd3473b8d998fce0a68e119
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_doaj_primary_oai_doaj_org_article_caf4e3785dd3473b8d998fce0a68e119
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ISSN
1474-760X,1474-7596
E-ISSN
1474-760X
DOI
10.1186/s13059-019-1783-3
